Journal: eLife

Article Title: An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis

doi: 10.7554/eLife.30126

Figure Lengend Snippet:

Article Snippet: antibody , Rat monoclonal anti-Jagged1 , DSHB (Iowa, USA) , Ts1.15h, RRID: AB_528317 , 1/300, IHC.

Techniques: Recombinant, Transfection, Protease Inhibitor, In Situ, Sequencing, Cloning, In Situ Hybridization, Construct, Generated, Plasmid Preparation, Mutagenesis, Software

Journal: OncoTargets and therapy

Article Title: Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling

doi: 10.2147/OTT.S40914

Figure Lengend Snippet: The effect of EGCG on expressions of Notch1, Notch2, HES1 , and JAG1 in the colorectal cancer cells: ( A ) the expression of HES1 ; ( B ) the expression of JAG1 ; ( C ) the expression of Notch1 ; and ( D ) the expression of Notch2 . Notes: The x-axis shows the Lovo, SW480, HT-29, and HCT-8 colorectal cell lines. The y-axis shows the mRNA of the colorectal cell lines after treatment with EGCG. An independent-sample t -test was used to analyze the mRNA levels of the colorectal cell lines. Abbreviation: EGCG, epigallocatechin gallate.

Article Snippet: The primary antibodies used were Abcam Hes1 antibody (ab71559), CST Jagged1 (28H8Rabbit mAb), CST Notch1 (D1E11 XP™ Rabbit mAb), and CST Notch2 (8A1Rabbit mAb) (Sigma-Aldrich, St Louis, MO, USA).

Techniques: Expressing

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Serelaxin promotes the preservation of endothelial cell properties in both in vivo and in vitro by regulating candidate genes. (A) Heat map and (B) scatter plot show the genes with altered mRNA expression level in only TGFβ1-treated (x-axis) or in both TGFβ1- and Serelaxin-treated (y-axis) HCAECs. STEAP1, NOTCH1, JAGGED1, RAC1, DSP, GSC, ERBB3, FZD7, GSK3B and TGFB2 were significantly regulated by Serelaxin treatment, which is shown by separation from dot lines (cut-off by 4 folds). (C-D) qPCR analysis showing the expression of candidate genes, which were identified from qPCR array in TGFβ1- and Serelaxin-treated MCECs. Cells without any treatment were used as control. (E) qPCR analysis of the panel of candidate genes in vivo in sham and AAC-operated mice treated with vehicle or Serelaxin. Serelaxin significantly rescued the effects of AAC on all genes except for Gsc and Steap1. Student t-test was used for single comparison and one-way ANOVA with Bonferroni post-hoc analysis was used for multiple group comparisons. Gene expression and associated error bars, representing mean ± SEM n≥3, n.s. no significance, * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Preserving, In Vivo, In Vitro, Expressing, Control, Comparison, Gene Expression

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Serelaxin rescues the Notch1 pathway in AAC-operated hearts. Immunofluorescence staining of (A) Jagged1, (B) Notch1 and (C) NICD in combination with WGA and DAPI in sham and AAC-operated hearts treated with vehicle or Serelaxin. Quantifications showed that protein expressions of (D) Jagged1, (E) Notch1 and (F) NICD were downregulated in AAC-operated hearts and could be restored by Serelaxin. (G) Western blot analysis shows the amount of the soluble form of Jagged1 in the medium of TGFβ1-treated MCECs. Ponceau-S stained membrane picture indicates that an equal amount of total precipitated protein was loaded for both the control and TGFβ1-treated MCECs. Soluble Jagged1 was reduced in TGFβ1-treated MCECs as quantified by densitometry analysis (right panel). Student t-test was used for single comparison and one-way ANOVA with Bonferroni post-hoc analysis was used for multiple group comparisons. Gene expression and associated error bars, representing mean ± SEM, n≥3, n.s. no significance, * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Immunofluorescence, Staining, Western Blot, Membrane, Control, Comparison, Gene Expression

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Antibodies used in Immunofluorescence staining

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Immunofluorescence

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Primers used in qPCR assay for gene expression analysis

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Gene Expression, Sequencing